Apparent Km and Vmax Calculator

Calculate Apparent Km and Vmax







What are Apparent Km and Vmax?

In the presence of an inhibitor, the apparent Km and Vmax are the modified values of the Michaelis constant and maximum reaction velocity, respectively.

Formulas

The formulas to calculate apparent Km and Vmax are:

\[ K_m^{\text{app}} = K_m \left(1 + \frac{[I]}{K_I}\right) \]

\[ V_{max}^{\text{app}} = \frac{V_{max}}{1 + \frac{[I]}{K_I}} \]

Example Calculation

Consider the following details:

Using the formulas:

\[ K_m^{\text{app}} = 10 \left(1 + \frac{5}{2}\right) = 35 \]

\[ V_{max}^{\text{app}} = \frac{100}{1 + \frac{5}{2}} = 28.57 \]