Concentration After Dilution Calculator

Formula

The formula to calculate the final concentration after dilution is:

\[ C2 = C1 \times \left(\frac{V1}{V2}\right) \]

Where:

C2 is the final concentration (ng/ul)
C1 is the initial concentration (ng/ul)
V1 is the initial volume (ul)
V2 is the final volume (ul)

What is a Ng/Ul Dilution?

A Ng/Ul dilution refers to the concentration of a particular substance in a solution, measured in nanograms (Ng) per microliter (Ul). This is commonly used in molecular biology and biochemistry to quantify the concentration of DNA, RNA, or protein in a sample. The dilution process involves reducing the concentration of the substance in the solution, often to make it suitable for a particular experiment or procedure.

Example Calculation

Let's assume the following values:

Initial Concentration (C1): 50 ng/ul
Initial Volume (V1): 100 ul
Final Volume (V2): 200 ul

Step 1: Calculate the final concentration using the formula:

\[ C2 = 50 \times \left(\frac{100}{200}\right) = 50 \times 0.5 = 25 \]

The final concentration is 25 ng/ul.