The DNA concentration is calculated using the following formula derived from the Beer-Lambert Law:
\[ C = \frac{A_{260}}{l} \times CF \times DF \]
Where:
\( C \) = Concentration of the nucleic acid in the sample
\( A_{260} \) = The maximum absorbance at 260 nm
\( l \) = Pathlength of the cuvette (standard is 1 cm)
\( CF \) = Conversion factor (33 µg/mL for ssDNA, 50 µg/mL for dsDNA, 40 µg/mL for RNA)
\( DF \) = Dilution factor (applies when the sample is diluted)