Dead Volume Calculator

Formula

The formula to calculate the Dead Volume is:

\[ V_d = V_t - (V_c + V_m) \]

Where:

\( V_d \) is the Dead Volume
\( V_t \) is the Total Volume
\( V_c \) is the Column Volume
\( V_m \) is the Mobile Phase Volume

What is Dead Volume?

Dead volume, also known as void volume, is the volume within a chromatography system that is not occupied by the stationary phase. It includes the volume of the mobile phase and any other components that do not interact with the stationary phase. Dead volume is an important parameter in chromatography as it affects the separation efficiency and resolution of the analytes. Minimizing dead volume is crucial for achieving accurate and reproducible results in chromatographic analyses.

Example Calculation

Let's assume the following values:

Total Volume (V_t) = 100 mL
Column Volume (V_c) = 30 mL
Mobile Phase Volume (V_m) = 20 mL

Using the formula:

\[ V_d = 100 - (30 + 20) = 50 \, \text{mL} \]

The Dead Volume (V_d) is 50 mL.