Ligation Molar Ratio Calculator

What is a Ligation Molar Ratio?

The ligation molar ratio is a critical parameter in molecular biology, particularly in the process of DNA ligation. This ratio represents the proportion of insert DNA to vector DNA in a ligation reaction. Achieving the correct molar ratio is essential for efficient ligation, as it influences the likelihood of the insert DNA being successfully incorporated into the vector DNA. A well-balanced molar ratio ensures that there are enough insert molecules to interact with the vector molecules, facilitating the formation of recombinant DNA molecules. This is a fundamental step in cloning, gene expression studies, and various genetic engineering applications.