The formula to calculate Partition Coefficient (K) is:
\[ K = \frac{c_{s}}{c_{m}} \]
Where:
The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.
The concentration of Solute in the Stationary Phase is the amount of solute and particles that are dissolved in a solution in the stationary phase.
The concentration of Solute in the Mobile Phase is the amount of solute and particles that are dissolved in a solution in the mobile phase.
Let's assume the following values:
Using the formula:
\[ K = \frac{c_{s}}{c_{m}} \]
Evaluating:
\[ K = \frac{87}{80} \]
The Partition Coefficient is 1.0875.
| Concentration of Solute in Stationary Phase (Mole per Cubic Meter) | Concentration of Solute in Mobile Phase (Mole per Cubic Meter) | Partition Coefficient |
|---|---|---|
| 80 | 70 | 1.142857142857143 |
| 80 | 75 | 1.066666666666667 |
| 80 | 80 | 1.000000000000000 |
| 80 | 85 | 0.941176470588235 |
| 80 | 90 | 0.888888888888889 |
| 82 | 70 | 1.171428571428571 |
| 82 | 75 | 1.093333333333333 |
| 82 | 80 | 1.025000000000000 |
| 82 | 85 | 0.964705882352941 |
| 82 | 90 | 0.911111111111111 |
| 84 | 70 | 1.200000000000000 |
| 84 | 75 | 1.120000000000000 |
| 84 | 80 | 1.050000000000000 |
| 84 | 85 | 0.988235294117647 |
| 84 | 90 | 0.933333333333333 |
| 86 | 70 | 1.228571428571429 |
| 86 | 75 | 1.146666666666667 |
| 86 | 80 | 1.075000000000000 |
| 86 | 85 | 1.011764705882353 |
| 86 | 90 | 0.955555555555556 |
| 88 | 70 | 1.257142857142857 |
| 88 | 75 | 1.173333333333333 |
| 88 | 80 | 1.100000000000000 |
| 88 | 85 | 1.035294117647059 |
| 88 | 90 | 0.977777777777778 |
| 90 | 70 | 1.285714285714286 |
| 90 | 75 | 1.200000000000000 |
| 90 | 80 | 1.125000000000000 |
| 90 | 85 | 1.058823529411765 |
| 90 | 90 | 1.000000000000000 |