The protein concentration is calculated using the following formula derived from the Beer-Lambert Law:
\[ C = \frac{A}{\epsilon \cdot b} \times m \times n \]
Where:
\( C \) = Protein concentration
\( A \) = Absorbance at \(\lambda_{\text{max}}\)
\( \epsilon \) = Extinction coefficient
\( b \) = Pathlength of the cuvette
\( m \) = Molecular mass
\( n \) = Dilution factor