The formula to calculate the Retention Factor (Rf) is:
\[ Rf = \frac{Dc}{Ds} \]
Where:
The Retention Factor, or Rf, is a measure used in thin-layer chromatography (TLC) to evaluate the progress of a chromatographic separation. It is a ratio of the distance a solute travels on the chromatogram relative to the distance the solvent front travels from the baseline. A lower Rf value indicates that the compound is less mobile in the solvent, while a higher Rf value suggests greater mobility. The Rf value can be used to identify compounds by comparing it to known standards.
Let's assume the following values:
Using the formula to calculate the Retention Factor (Rf):
\[ Rf = \frac{Dc}{Ds} = \frac{5}{10} = 0.50 \]
The Retention Factor (Rf) is 0.50.