The formula to convert µg/mL to µM is:
\[ \mu M = \left( \frac{\mu g/mL}{MW} \right) \times 1000 \]
Where:
Converting µg/mL to µM is a common task in chemistry and biology, particularly in the context of preparing solutions for experiments. The concentration in µg/mL measures the mass of a substance in micrograms present in one milliliter of solution, while µM is a measure of the number of moles of a substance in one liter of solution. This conversion is necessary when the molecular weight of the substance is known and one needs to express concentration in terms of molarity, which is a more useful unit in chemical reactions.
Let's assume the following values:
Step 1: Divide the concentration by the molecular weight:
\[ \frac{50}{180} = 0.2778 \]
Step 2: Multiply by 1000 to convert to micromolar:
\[ \mu M = 0.2778 \times 1000 = 277.8 \text{ µM} \]
Therefore, the concentration is 277.8 µM.
Let's assume the following values:
Step 1: Divide the concentration by the molecular weight:
\[ \frac{100}{150} = 0.6667 \]
Step 2: Multiply by 1000 to convert to micromolar:
\[ \mu M = 0.6667 \times 1000 = 666.7 \text{ µM} \]
Therefore, the concentration is 666.7 µM.