PCR Amplification Calculator

Formula

To calculate the Final Amount of DNA (FA):

\[ FA = I \times (1 + E)^C \]

Where:

\(FA\) is the Final Amount of DNA (ng)
\(I\) is the Initial Amount of DNA (ng)
\(E\) is the Efficiency of the PCR Reaction
\(C\) is the Number of Cycles

What is PCR Amplification?

PCR (Polymerase Chain Reaction) Amplification is a laboratory technique used to make multiple copies of a specific segment of DNA. It is highly precise and capable of copying a specific DNA sequence billions of times in just a few hours. This process is crucial in genetic testing, criminal forensics, and molecular biology research, as it allows for the analysis of very small amounts of DNA.

Example Calculation

Let's assume the following values:

Initial Amount of DNA (\(I\)) = 10 ng
Efficiency of PCR Reaction (\(E\)) = 1
Number of Cycles (\(C\)) = 30

Using the formula:

\[ FA = 10 \times (1 + 1)^{30} = 10 \times 2^{30} = 10 \times 1073741824 = 10737418240 \text{ ng} \]

The Final Amount of DNA is 10,737,418,240 ng.