PCR Annealing Temperature Calculator

The PCR annealing temperature is calculated using the following empirical formula:

\[ T_a^* = 0.3 \cdot T_{mp} + 0.7 \cdot T_{mt} - 14.9 \]

Where:

\( T_a^* \) = Optimal annealing temperature

\( T_{mp} \) = Melting temperature of the primer

\( T_{mt} \) = Melting temperature of the target DNA

Inputs