Sequencing Coverage Calculator

Formula

To calculate the sequencing coverage:

\[ \text{Sequencing Coverage} = \frac{\text{Read Length} \times \text{Number of Reads}}{\text{Genome Size}} \]

Where:

\(\text{Sequencing Coverage}\) is the average number of times each base in the genome is sequenced
Genome Size is the total size of the genome in base pairs (bp)
Read Length is the length of each sequencing read in base pairs (bp)
Number of Reads is the total number of sequencing reads

What is Sequencing Coverage?

Sequencing coverage refers to the average number of times each base in a genome is sequenced. Higher coverage increases the accuracy and reliability of the sequencing data.

Example Calculation

Let's assume the following values:

Genome Size = 3,000,000,000 bp
Read Length = 150 bp
Number of Reads = 100,000,000

Using the formula:

\[ \text{Sequencing Coverage} = \frac{150 \times 100,000,000}{3,000,000,000} = 5 \text{x} \]

The sequencing coverage is 5x.